Fig. 3

PICs are clonogenic and clones can be maintained over long-term culture. a Representative light microscope image of a single PIC in a well of a 96-well plate (scale bar = 100 μm), and subsequent clonal population after 12 days of culture (scale bar = 500 μm). b Representative light microscope images of C9 PIC morphology at passage (P)1, P10, and P20. Scale bar = 200 μm. c,d Flow cytometric analysis of C9 PICs for Sca-1 (c) and PW1 (d) at P2, P10, and P20. e Population doubling time of C9 PICs over 20 passages. Data are mean ± SD, n = 3. f qRT-PCR transcript analysis of C9 PICs at P1, P10, and P20. Bars represent the mean relative expression normalised to GAPDH. Error bars represent the standard deviation of the mean; n = 3