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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Different therapeutic effects of cells derived from human amniotic membrane on premature ovarian aging depend on distinct cellular biological characteristics

Fig. 5

hAMSCs upregulated the expression level of immune molecules in hPBMCs more forcefully than hAECs. a Schematic overview of hPBMC filter procedures. b Expression level of CD8 in hPBMCs evaluated by FACS after coculture with hAECs and hAMSCs respectively. c Expression level of CD4 in hPBMCs evaluated by FACS after coculture with hAECs and hAMSCs respectively. d Expression level of CD11b in hPBMCs evaluated by FACS after coculture with hAECs and hAMSCs respectively. e Expression level of CD19 in hPBMCs evaluated by FACS after coculture with hAECs and hAMSCs respectively. f Expression level of CD56 in hPBMCs evaluated by FACS after coculture with hAECs and hAMSCs respectively. Experiments were carried out after 7 days of coculture, n = 3. Error bars indicate SD. *p < 0.05, **p < 0.01, ***p < 0.001, compared with control group; #p < 0.05, ##p < 0.01, compared with hAEC group. Sal saline, DOR decreased ovarian reserve, POF premature ovarian failure, hPBMC human peripheral blood mononuclear cell, hAEC human amniotic epithelial cell, hAMSC human amniotic mesenchymal stem cell, ​MFI mean fluorescence intensity

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