Fig. 5

Characterization of precursors to insulin-producing cells (pIPCs). a Fold change in the expression of pancreatic lineage genes in pIPCS and insulin-producing cells (IPCs). The controls for calculating the fold changes in pIPCs were passage 0 cells. However, for calculating the fold change in IPCs, the controls were uninduced cells which were derived from late passages, like pIPCs (n = 3). b Western blots showing the expression of PDX1 protein at 46 kDa and loading control HSP90 at 90 kDa. Lane 1 is the molecular weight marker (M), lane 2 shows the cell lysate of the bone marrow-derived CD45-negative cell population as negative control (CD45-), lane 3 is empty (EL), lane 4 is pancreatic lysate (PL) as a positive control, followed by lysates from passage (P)0, 10, 11, and 13. C is the lysate from uninduced triple positive control cells, and I is the lysate from induced and differentiated IPCs. c–e Dendograms showing flow cytometric analysis for the percentage of PDX1-positive cells in pIPCs in a representative sample: c unstained MSCs; d PDX-1-FITC-stained cells; e a merge of PDX-1-stained and -unstained cells show that 60.4% of MSCs grown in high-glucose media were positive for PDX1. 78.65 ± 10.31% (mean ± SEM) cells were positive for PDX-1 (n = 3)