Fig. 2
From: Isolation and characterization of equine endometrial mesenchymal stromal cells
Isolation and culture of MSCs. a Micrograph showing cells cultured directly following digestion of equine endometrium. Using this procedure, clusters of epithelial cells (black arrows) eventually outgrew stromal cells in culture. b Section of equine endometrium stained for Mucin-1, showing positive cells in epithelia and glands (white arrows). Cell nuclei stained with DAPI. c Micrograph of endometrial stromal cells in culture obtained after separation of epithelial cells (shown in d) using beads bound to Mucin-1. e Cell colonies produced after seeding of endometrial and BM MSCs at low densities. f Cloning efficiencies (CE) for endometrial MSCs (n = 6 horses) and BM MSCs (n = 3 horses) at two different seeding densities. Scale bars: 1 mm (a, c–e). BM bone marrow, MSC mesenchymal stromal/stem cell (Color figure online)