Fig. 5

Trilineage differentiation of endometrial MSCs (a, c, e) and BM MSCs (b, d, g). Representative images of endometrial and BM MSCs (n = 3 horses each) after differentiation and staining with Oil red O (a, b), Alizarin Red (c, d) and Alcian Blue/Nuclear Fast Red (e–g) to assess differentiation into adipogenic, osteogenic, and chondrogenic lineages, respectively. Insets show nondifferentiated control cells (a–d). Nondifferentiated control BM MSCs used in chondrogenic differentiation experiments shown in (f). Scale bars: 100 μm (a, b), 500 μm (c, d, e, g) and 1 mm (f)