Fig. 8

Role of FAK and Rho GTPases on F-actin organization after Ang II stimulation. a Immunofluorescence analysis of F-actin polymerization performed using rhodamine phalloidin (F-actin; red–orange). Nuclei were stained with DAPI (blue). An overlay of the two fluorescent signals is shown (scale bars = 20 μm). b–d A colorimetric ELISA-based assay was used to estimate the Rho GTPase activity levels in cell lysates. Statistically significant differences are indicated (n = 3; *p < 0.05 vs normal, ^# p < 0.05 vs the control group). Ang II angiotensin II, OD optical density, DAPI 4', 6-diamidino-2-phenylindole