Fig. 2
Effect of IL-3 on wound healing and cell motility of human MSCs. BM-MSCs, AT-MSCs and GT-MSCs (104 cells/well) were seeded in 24-well culture plates. After 80–90% confluency, wounds were created on monolayers using a 200 μl pipette tip. Cells were washed and incubated for 18 hours in the absence or presence of human IL-3 (100 ng/ml). Representative images of human BM-MSCs (a), AT-MSCs (b) and GT-MSCs (c) at 0 and 18 hours of IL-3 treatment (Magnification 10×). Percent wound closure from three independent experiments was analyzed (d). Human BM-MSCs, AT-MSCs and GT-MSCs were incubated for 24 hours with and without IL-3 and cell motility was examined by measurement of accumulated (e) and euclidean (f) distance travelled by MSCs. The cell motility images were captured by time-lapse microscope and analyzed using Image J Software. Data shown as mean ± SEM of three independent experiments. *p ≤ 0.05 and ***p ≤ 0.001 vs control group. AT adipose tissue, BM bone marrow, CTRL control, GT gingival tissue, IL-3 interleukin-3, MSC mesenchymal stem cell