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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Interleukin-3 enhances the migration of human mesenchymal stem cells by regulating expression of CXCR4

Fig. 6

IL-3-treated MSCs migrate towards SDF-1α. IL-3-pretreated (100 ng/ml) and untreated BM-MSCs and AT-MSCs were seeded in the upper chamber of cell culture inserts and SDF-1α (10, 30, 60 ng/ml) was added to the lower chamber. After 18 hours, migration of cells towards SDF-1α was visualized by staining the cells from the lower side of inserts with hematoxylin (a, b). Cells from the lower side were also removed by trypsinization and counted (c). IL-3-pretreated and untreated BM-MSCs and AT-MSCs were seeded in the upper chamber of cell culture inserts with or without AMD3100 (10 μM) and SDF-1α (60 ng/ml) was added to the lower chamber. After 18 hours, cells from the lower side of inserts were trypsinized and counted (d). Wounds created on monolayers of BM-MSCs and AT-MSCs were incubated with IL-3 in the presence or absence of AMD3100 and percent wound closure was analyzed (e). Data are representative of two independent experiments. *p ≤ 0.05, **p ≤ 0.01 and***p ≤ 0.001 vs control group or vs IL-3-pretreated group. AT adipose tissue, BM bone marrow, CTRL control, IL-3 interleukin-3, MSC mesenchymal stem cell, SDF-1α stromal cell-derived factor-1 alpha

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