Table 1 A list of osteoblast, adipocyte, and chondrocyte primers
|  | Primer | Forward (5’–3’) | Reverse (5’–3’) |
|---|---|---|---|
Osteoblast markers | BMP2 | CCCTACATGCTAGACCTGTATC | GTTGTTTTCCCACTCGTTTCTG |
OPN | CCTTCCAAGTAAGTCCAACGAA | GACAACTGGAGTGAAAACTTCG | |
OCN | ATGAGAGCCCTCACACTCCTC | GCCGTAGAAGCGCCGATAGGC | |
ALP | TGGAGCTTCAGAAGCTCAACACCA | ATCTCGTTGTCTGAGTACCAGTCC | |
RUNX2 | TTTAGGGCGCATTCCTCATC | GGAGGGCCGTGGGTTCT | |
Osteocyte markers | FGF23 | TTGGATCACACTATTTCGACCC | GAAGTGAATTAGGGGGATCTCG |
DMP1 | TCTTTGTGAACTACGGAGGGTA | TGAGCCAAATGACCCTTCCA | |
MEPE | GAGGAAAAGGTAGACTGAGATTCT | GGGACAAATCTTTCTTTCTTTCCT | |
SOST | CAAGAATGATGCCACGGAAATC | GGACACGTCTTTGGTCTCAA | |
Control | RPLP0 | CAGCAAGTGGGAAGGTGTAATCC | CCCATTCTATCATCAACGGGTACAA |
Adipogenic markers | ACAN | TGATGTTCCCTGCAATTACCAC | CAAAAAGCGACAAGAAGAGGAC |
Leptin | ATTTTCAGAAGAGAACGGACATTC | TGCTCCCCTTCTTCAAAATGTA | |
Chondrogenic markers | ATP2A2 | AACTACCTGGAACCTGCAATAC | GGGTTGGTAGATGTGTTGCTAA |
COL10A1 | GAGTAAAGGTATAGCAGTAAGAGGA | CATATGGTCCTCTCT CTCCTGG | |
PPRAG | AAGACAACAGACAAATCAACCG | GTCTTCTTGATCACCTGCAGTA |