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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Human dental pulp pluripotent-like stem cells promote wound healing and muscle regeneration

Fig. 6

Histological, morphometric and fibre type analyses after DPPSC injection in dystrophic Sgcb-null Rag2-null γc-null mice. a, b Haematoxylin and eosin staining in control (a) or DPPSC-injected (b) Sgcb-null Rag2-null γc-null muscles. Scale bars: 100 μm. c Quantitative frequency distribution analysis of the cross-sectional area of the fibres in Sgcb-null Rag2-null γc-null muscles. *p < 0.05, n = 3 for each group. d, e Masson’s trichrome staining in control (d) or DPPSC-injected (e) Sgcb-null Rag2-null γc-null muscles revealing areas of fibrosis (blue). Scale bars: 100 μm. f Quantitative analysis of the percentage of fibrosis per field in Sgcb-null Rag2-null γc-null muscles. *p < 0.05, n = 4 for each group. g, h Sirius Red staining visualised by bright field microscopy in control (g) or DPPSC-injected (h) Sgcb-null Rag2-null γc-null muscles for the analysis of total collagen. Scale bars: 100 μm. i Quantification of the total collagen present in Sgcb-null Rag2-null γc-null muscles. *p < 0.05, n = 4 for each group. j, k NADH staining of control (j) and DPPSC-injected (k) Sgcb-null Rag2-null γc-null muscles, showing oxidative fibres in blue and glycolytic fibres in white. Scale bars: 100 μm. l, m Quantitative frequency distribution analysis of the cross-sectional area of type II fast-glycolytic (l) or type I slow-oxidative (m) fibres in the NADH staining in Sgcb-null Rag2-null γc-null muscles injected with DPPSC compared to control muscles. *p < 0.05, n = 3 for each group. For c, f, i, l, m, two-tailed Student’s t test was used and results are displayed as mean ± s.e.m

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