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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Minocycline modulates NFκB phosphorylation and enhances antimicrobial activity against Staphylococcus aureus in mesenchymal stromal/stem cells

Fig. 7

Internalization of SA by MSCs and complement system activation. a Confocal images of SA internalization by MSCs after 4 hours of MSC and SA coculture on glass coverslips indicating MSC nuclei (DAPI), MSC cell bodies (actin green) and SA (vancomycin BODIPY) (400× magnification, scale bar 800 μm). b Quantification of SA internalization by MSCs. c Colony forming abilities of SA after 6 hours of cocultures including MSC treatments with minocycline and internalization inhibitors chloroquine or 3-methyladenine. C5 (d), C5a (e), C3 (f), and C3b (g) protein levels in MSC and SA cocultures. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001. CFU colony forming unit, MSC mesenchymal stromal/stem cell, SA Staphylococcus aureus, TNF-α tumor necrosis factor alpha, TSB tryptic soy broth

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