Skip to main content

Table 1 CD34 expression on day 7 after treatment with different small-molecule combinations

From: A small-molecule/cytokine combination enhances hematopoietic stem cell proliferation via inhibition of cell differentiation

Groups

Medium content

CD34 (%)

Small molecules

Cytokines

SMC

SR1 + VPA

TSF

90.5 ± 0.5*#†‡

SR1 + C433

 

86.6 ± 12.5*#§

SR1 + VPA + C433

 

94.5 ± 4.3*#

VC

DMSO

TSF

56.6 ± 1.8

39.8 ± 0.7

52.2 ± 1.7

SMCO

SR1 + VPA

Absent

N/A

SR1 + C433

SR1 + VPA + C433

NC

Absent

TSF

45.5 ± 0.2

BC

Absent

Absent

N/A

  1. The cells were not enough for the flow cytometry detection of the SMCO group
  2. Data are shown as mean ± SD (n = 3)
  3. *p < 0.01, SMC vs VC; # p < 0.01, SMC vs NC; † p < 0.01, SR1 + VPA vs SR1 + C433; ‡ p < 0.05, SR1 + VPA vs SR1 + VPA + C433; § p < 0.05, SR1 + C433 vs SR1 + VPA + C433
  4. BC basal control, C433 CAY10433, DMSO dimethyl sulfoxide, N/A not applicable, NC negative control, SMC small-molecule combination, SMCO small-molecule combination only, SR1 stemregenin1, TSF TPO + SCF + Flt3-L, VC vehicle control, VPA valproic acid