Fig. 1
From: Culturing and transcriptome profiling of progenitor-like colonies derived from adult mouse pancreas
Single cells from adult mouse pancreas could form ring colonies. a Single cells from adult mouse pancreas formed ring colonies after 2 weeks of culture in 3D medium. Scale bars, 200 μm. b Colony-forming frequency with/without certain factors in medium. EGF and Nicotinamide were pivotal for colony-forming. c Self-renewal capacity tested by serial passaging. d Pancreas-related genes analyzed by real-time PCR. Two-week-old ring colonies (n = 7) enriched pancreatic ductal and progenitor genes. ***p < 0.005. EGF epidermal growth factor, Sox9 sex determining region Y-box 9, Ck7 cytokeratin 7, Ngn3 Neurog 3, Ptf1a pancreas specific transcription factor 1α, Nkx6.1 NK6 homeobox 1, Nkx2.2 NK2 homeobox 2, Mafa v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A, Pax4 paired box 4, Cyc cyclophilin (Color figure online)