Fig. 2
From: Culturing and transcriptome profiling of progenitor-like colonies derived from adult mouse pancreas
Immunostaining of pancreatic progenitor-related transcriptional factors in ring colonies. a–d Whole-mount immunostaining of single ring colonies showed PDX1/Ki67, CK7, SOX9 and NEUROG3 expression. Scale bars, 50 μm. PDX1/Ki67 coexpression indicated a proliferative pancreatic progenitor-like phenotype. CK7 and SOX9 showed the ductal origin of the colony. Weak expression of NEUROG3 implied its potential for endocrine lineage. SOX9 sex determining region Y-box 9, CK7 cytokeratin 7, NGN3 neurog 3, PDX1 Pancreatic and duodenal homeobox 1 (Color figure online)