Fig. 3
From: Culturing and transcriptome profiling of progenitor-like colonies derived from adult mouse pancreas
Cells from ring colonies can be induced to differentiate to beta lineage. a After enrichment in a 3D system, colonies after three passages were dissolved and plated in a 2D system for differentiation. Scale bars, 200 μm. b Plaques formed in a 2D system and cells reached nearly 100% confluence in 5 days. c Immunostaining showing expression of CK7, PDX1 and Ki67 in 2D plaques. Scale bars, 100 μm. d NEUROG3 detected after 1 week of culture in differentiation medium, indicating the commitment of endocrine progenitors. Scale bars, 200 μm. e–g Clusters found at day 14 in differentiation medium and the staining of DTZ and C-peptide was positive. Scale bars, 50 μm. C-pep C-peptide, CK7 cytokeratin 7, DTZ Dithizone, PDX1 Pancreatic and duodenal homeobox 1 (Color figure online)