Fig. 1
From: Extracellular vesicles from human liver stem cells restore argininosuccinate synthase deficiency
Characterization of ASS1-HLSCs. a Representative flow cytometric analysis of markers expressed by ASS1-HLSC (black histograms; dotted histograms represent the isotypic controls). Cells were labeled with FITC- or PE-conjugated antibody. One hundred percent of ASS1-HLSCs were positive for CD90, CD73, CD29, and CD105, and negative for CD45 as described previously for normal HLSCs [4]. ASS1-HLSCs were also positive for the liver marker albumin [4]. Data represent one of three experiments with similar results. b Representative immunofluorescence micrographs of ASS1-HLSCs stained with antibodies against albumin, nestin, α-fetoprotein (α-FP), nanog, sox2, vimentin, cytokeratin (CK)8 and CK19. Hoechst dye 33258 was applied for nuclei staining. Scale bars = 5 μm. Data represent one of three experiments with similar results