Fig. 2
From: Extracellular vesicles from human liver stem cells restore argininosuccinate synthase deficiency
The expression of ASS1 gene and ASS1 mRNA in HLSCs and ASS1-HLSC. a,b Representative SNaPshot sequences of ASS1 gene in normal human liver stem cells (HLSCs) and HLSCs from the liver of a patient suffering from citrullinemia type 1 (ASS1-HLSCs). a The upper electropherogram shows a normal ASS1 gene profile from HLSCs; the lower electropherogram shows the mutated gene profile from ASS1-HLSCs (c.1087C > T). b The upper electropherogram shows a normal ASS1 gene profile; the lower electropherogram shows the mutated ASS1-HLSC gene profile (c.168G > A). Data represent one of three experiments with similar results. c qRT-PCR analysis showing the expression of ASS1 isoforms 1 (Iso1) and 2 (Iso2) mRNA in human hepatocytes (hH), HLSCs, and ASS1-HLSCs. hH were used as positive control. d qRT-PCR analysis of ASS1 Iso1 mRNA in HLSCs transfected with ASS1 shRNA. Data represent the mean of two independent experiments performed in triplicate. *p < 0.05, HLSC shRNA-ASS1 vs HLSC Ctrl-SCR. A adenine, C cytosine, F.U fluorescence unit, G guanine, SCR scramble, T thymine