Fig. 8

ASS1 mutations. a,b SNaPshot method was used to characterize the mutations in human liver stem cells (HLSCs) from the liver of a patient suffering from citrullinemia type 1 (ASS1-HLSCs). ASS1-HLSCs were treated with 1 × 10¹⁰ HLSC-derived extracellular vesicles (EVs) in adherence culture conditions for 6, 24, and 48 h. At the end of the experiments, DNA was recovered from ASS1-HLSCs treated with or without EVs and sequencing mutation experiments were performed to detect C-T mutations (a) and G-A mutations (b). Data are expressed as mean ± SD of three individual experiments. ANOVA with Dunet’s multicomparison tests was performed. *p < 0.05, all experimental conditions vs ASS1-HLSC. c–d Representative electropherograms of ASS1-HLSCs performed to detect C-T mutation (c) and G-A mutation (d). e-f Representative electropherograms of ASS1-HLSCs treated for 6 h with 1 × 10¹⁰ HLSC-EVs showing the reduction of C-T mutation (e) and G-A mutation (f). A adenine, C cytosine, F.U fluorescence unit, G guanine, T thymine