Table 1 Primers used in qRT-PCR to evaluate ASS1 mRNA expression
From: Extracellular vesicles from human liver stem cells restore argininosuccinate synthase deficiency
PCR primers | Primer sequences (5′–3′) |
|---|---|
hASS1-Iso1 | F1 TGT GAA AAC AGA TTC CACG C |
R1 CCA ATG TTG GCC AGA TAG GC | |
hASS1-Iso2 | F1 ACG CTA TGT CCA GCA AAG GC |
R1 CCA ATG TTG GCC AGA TAG GC | |
hASS1-Exon 14 (Primer 1) | F AAGTCCCAGGAGCGAGTGG |
R GTGGGGCACCTACCTCACC | |
hASS1-Exon 15 (Primer 2) | F GCCAGGCTGAGCTGACAAG |
R ACCTGGAGGCTCTGAAGGC |