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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Inhibitory effects of HNF4α on migration/maltransformation of hepatic progenitors: HNF4α-overexpressing hepatic progenitors for liver repopulation

Fig. 2

HNF4α overexpression suppressed the proliferation and migration of hepatic oval cells. a Recombinant HNF4α-overexpression plasmid and empty EGFP-N1 vector. b Forty-eight hours post transfection of HNF4α or EGFP-N1 plasmids, EGFP fluorescence was detected in some hepatic oval cells. c HNF4α-overexpressing hepatic oval cells isolated by flow cytometry. d RT-PCR data revealed that HNF4α-overexpressing hepatic oval cells showed more transcription of HNF4α and ALB, yet less transcription of CCND1 and PCNA. e Western blot analysis confirmed that HNF4α-overexpressing hepatic oval cells expressed more HNF4α and ALB, yet less PCNA. f Wound closure was photographed and evaluated at 0 and 24 hours post scratching of HNF4α-overexpressing cells and EGFP-N1 control cells. Overexpression of HNF4α reduced the speed of wound closure when compared to EGFP-N1 control cells. ALB albumin, CCND1 cyclin D1, HNF hepatic nuclear factor, PCNA proliferating cell nuclear antigen (Color figure online)

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