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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Inhibitory effects of HNF4α on migration/maltransformation of hepatic progenitors: HNF4α-overexpressing hepatic progenitors for liver repopulation

Fig. 3

HNF4α suppression accelerated the proliferation and migration of hepatic oval cells. a Morphology of hepatic oval cells at 4 days post transfection of noneffective control shRNA and HNF4α shRNA). b RT-PCR data showed that HNF4α shRNA transfected cells expressed less HNF4α and ALB, but more CCND1 and PCNA than noneffective control shRNA transfected cells. c Western blot analysis confirmed that HNF4α shRNA transfected cells expressed less HNF4α and ALB, but more PCNA than noneffective control shRNA transfected cells. d Speed of wound closure was accelerated in HNF4α shRNA transfected cells when compared to noneffective control shRNA transfected cells. e Hepatic oval cells transfected with noneffective control shRNA did not proliferate in soft agar after 2-week cultivation, but HNF4α shRNA transfected cells formed some tiny clones in soft agar, although much smaller than HepG2 cells. ALB albumin, CCND1 cyclin D1, HNF hepatic nuclear factor, PCNA proliferating cell nuclear antigen

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