Fig. 2

hAdSCs reduced doxorubicin sensitivity by increasing ABCG2 protein expression and doxorubicin efflux in TNBC. a Doxorubicin (250 nM) reduced cell viability in both L15 and DMEM time-dependently. CM collected from hAdSC made MDA-MB-231 cells less sensitive to doxorubicin-induced cell death markedly, as measured by crystal violet staining (b) and SRB assay (c). d-f Protein expression of ABCG2, but not MRP-1 or P-Gp, was significantly upregulated by hAdSCs’ CM. g CM collected from hAdSCs reduced intracellular doxorubicin fluorescence measured by flow cytometric analysis, and blockage of ABCG2 by Ko143 antagonized hAdSCs’ CM-induced doxorubicin efflux in MDA-MB-231 cells. Graphs showed mean ± SD of three independent experiments. *p < 0.05; **p < 0.01; *p < 0.001 to control group. ^# p < 0.05 to doxorubicin in DMEM group. CM conditioned medium, DMEM Dulbecco’s modified Eagle’s medium, doxo doxorubicin