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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Mesenchymal stem cells derived from inflamed dental pulpal and gingival tissue: a potential application for bone formation

Fig. 1

Colony-forming unit assays and monolayer subculture. a Representative images of pulpal (DPSCs) and gingival (GMSCs) mesenchymal stem cell colonies (P0) and monolayer subcultures (P1), isolated from periodontally affected (P) and healthy (H) patients and stained with crystal violet. b Colony-forming assay (CFU-F) in dental mesenchymal stem cells. c Cell growth curve of P-DPSCs and H-DPSCs by trypan blue viability assay (P2). d, e The typical fibroblast-like cell shape of a monolayer subculture of P-DPSCs and H-DPSCs. f The bar plot represents the comparative cell cycle distribution analysis between P-DPSCs and H-DPSC (G2M + S = proliferation index (PI)). g Cell growth curve of P-GMSCs and H-GMSCs by trypan blue viability assay. h, i The typical fibroblast-like cell shape of a monolayer subculture of P-GMSCs and H-GMSCs at 72 h. l The bar plot represents the comparative cell cycle distribution analysis between P-GMSCs and H-GMSC (G2M + S = PI). Cell cycle analysis at P4. *p < 0.05. n.s. not significant

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