Fig. 3

Stem cell phenotyping by flow cytometry. a A representative figure of the cytometry analysis in periodontally affected (P) pulpal (DPSC) or gingival (GMSC) mesenchymal stem cells (P-DPSCs and P-GMSCs, respectively) and their healthy controls (H-DPSCs and H-GMSCs). In the same histogram graph, the green peak shows the positive cells and the purple peak is the unstained cells used as negative control. (Control: Iisotype controls were anti-IgG1 for CD34 and CD45, and anti-IgG2 for HLA-DR.) b Scatter dot plot graphs represent expression levels of the mesenchymal stem cell markers Stro-1, CD146, CD29, and SSEA4 (culture passage P5)