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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Mesenchymal stem cells derived from inflamed dental pulpal and gingival tissue: a potential application for bone formation

Fig. 5

Two cytokines promote H-GMSC and H-DPSC expansion. a Cell growth curve of healthy dental pulp mesenchymal stem cells (H-DPSCs) and healthy gingival MSCs (H-GMSCs) with and without cytokines (cyt; 20 ng/ml interleukin-1beta (IL1β) and 40 ng/ml tumor necrosis factor alpha (TNFα) for 72 h) by trypan blue viability assay. b Cytotoxicity assay: H-DPSCs and H-GMSCs without and after cytokine treatment. c qPCR analysis of cell cycle regulators and proliferation markers ccnd1, cdnk1b, and c-myc in H-DPSCs + cyt and H-GMSCs + cyt and their healthy controls at 24, 48, and 72 h. d Comparative analysis of nuclear and surface marker expression in H-DPSCs + cyt and H-GMSCs + cyt and their healthy controls at 72 h. Bone marrow MSCs (BM-MSCs) used as positive control (relative gene expression value = 1); β-actin is the housekeeping gene. Mean values ± SD of all samples studied are reported. SD bars are based on three independent experiments. Culture passage P5. *p < 0.05. FC fold change, n.s. not significant

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