Table 2 The primer sequence list used for the amplification of mesenchymal stem cell cDNA
Gene | Primer sequence | Code number |
|---|---|---|
ABCG2 | QT00073206 | |
CD105 | QT0001335 | |
THY-1 | QT00023569 | |
CD73 | QT00027279 | |
NANOG | QT01844808 | |
OCT4 | QT00210840 | |
SOX2 | F:5'- GGAGACGGAGCTGAAGCCGC-3' R:5'GACGCGGTCCGGGCTTGTTTT-3' | MWG |
IL-1β-R1 | F:5'-CCAGGGAACTATTTTTATTTTCTGG-3' F:5'-CTGAGAAGCTGGACCCCTTG-3' | MWG |
TNF-R1 | F:5'-GGGATAAAAGGCAAAGACCAA-3' F:5'-TCCTTCACCGCTTCAGAAAA-3' | MWG |
ccnd1 | QT00495285 | |
cdkn1b | QT00998445 | |
c-myc | F:5'-AAACACAAACTTGAACAGCTAC-3' F:5'-ATTTGAGGCAGTTTACATTATGG-3' | MWG |
runx-2 | F:5'-TACGACTGGACGCTGGTGC-3' R:5'-TTCATGGGTCGCTTGACGT-3' | MWG |
opn | F:5'-TGTGGGTTTCAGCACTCTGGTCA-3', R:5'-AAGCGAGTTGAATGGTGC-3' | MWG |
ocn | F:5'-CTGACCTCACAGATGCCAAG-3' R:5'-GTAGCGCCGGAGTCTGTTC -3' | |
CFL-1 (cofilin) | F:5'-TGCGGCTCCTACTAAACGG-3' F:5'-ACGCACCTTCATGTCGTTGA-3' | MWG |
PFN-1 (profilin) | F:5'-ACCCGGAAACAAGAAGAC-3' F:5'-ACTGGTCCGATAACCTCCCA-3' | MWG |
VCL (vinculin) | F:5'-ATGTCTCCTATATCCTGGTTT-3' F:5'-GCAGGAAGTGTCCTTCAGAC-3' | MWG |
HSPA9 (mortalin) | F:5'-TACAGCAGATGGTGAGCGAC-3' R:5'-TGCTGTGTGCCCCAAGTAAT-3' | MWG |
TXN-1 (thioredoxin-1) | F:5'- GTGAAGTCAAATGCACGCCA-3' R:5'-GCAGATGGCAACTGGTTATGT-3' | MWG |
HSP90AA1 | F:5'-GTGAAGTCAAATGCACGCCA-3' R:5'-GCAGATGGCAACTGGTTATGT-3' | MWG |