Fig. 2
Effects of the secretome with different concentrations of culture pO2 on hepatic recovery in partially hepatectomized mice. a (Left) Ki67 immunohistochemistry of liver specimens obtained from the mice injected with the secretome with different concentrations of culture pO2 at day 2 after PH. (Right) Percentage of Ki67-positive cells in each group at day 2 after injection. Of the secretome-injected groups, the number of Ki67-positive cells was highest in the liver specimens injected with the secretome of culture 1% pO2, followed by that with culture 21%, 5%, and 10% pO2, in that order. b Estimation of liver regeneration rates by LW/BW in each group at day 7 after injection. Of the secretome-injected groups, injection with the secretome of 1% pO2 exhibited the highest LW/BW, followed by injection with the secretome of 21%, 10%, and 5% pO2, in that order. c ELISA for the serum levels of IL-6 and TNF-α in each group at day 2 after injection. Of the secretome-injected groups, the serum levels of IL-6 and TNF-α were most significantly decreased in the 1% pO2 secretome group. Each group included five mice (25 mice in total). Data show means and SD for three independent experiments. *p < 0.05 compared to control. Ct control, IL interleukin, LW/BW ratio of liver weight to body weight, PH partial hepatectomy, pO 2 oxygen partial pressure, S21% secretome of culture 21% pO2, S10% secretome of culture 10% pO2, S5% secretome of culture 5% pO2, S1% secretome of culture 1% pO2, TNF-α tumor necrosis factor alpha