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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Protein deubiquitinase USP7 is required for osteogenic differentiation of human adipose-derived stem cells

Fig. 1

Protein deubiquitinase USP7 is potentially associated with hASC osteogenesis. a Schematic diagram of OC pro-Luc-hASC construction. b OC pro-Luc-hASCs were transfected with siRNA against USP7, with the scrambled siRNA as negative control and siRNA against RUNX2 as positive control. Left panel: Luciferase reporter assay with cellular extracts from OC pro-Luc-hASCs cultured in proliferation or osteogenic media for 7 days. Middle and right panel: Validation of USP7 and RUNX2 knockdown effect by Western blotting, respectively. c Relative mRNA expression of USP7 and RUNX2 measured by qRT-PCR during osteogenic differentiation of hASCs (normalized by GAPDH; relative to day 0 groups). d Western blotting of protein expression of USP7, RUNX2, and the internal control GAPDH during osteogenic differentiation of hASCs. Results are presented as the mean ± SD, n = 3. *P < 0.05, **P < 0.01. hASC human adipose-derived stem cell, Luc luciferase, NC negative control, OC osteocalcin, OM osteogenic media, PM proliferation media, RUNX2 runt-related transcription factor 2, USP7 ubiquitin specific protease 7

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