Fig. 4

USP7 inhibitor HBX 41,108 compromises osteogenic differentiation of hASCs. a Images of ALP staining on day 7, and AZR staining on day 14 of hASCs treated with proliferation or osteogenic media in the presence of vehicle or HBX 41,108. b Histograms show ALP activity and quantification of AZR staining by spectrophotometry. c qRT-PCR analysis of osteogenesis associated genes RUNX2, ALP, OC, and OSX expression in the presence of vehicle or HBX 41,108 on day 14 after osteoinduction. GAPDH was used for normalization. Results are presented as the mean ± SD, n = 3. *P < 0.05, **P < 0.01. ALP alkaline phosphatase, AZR alizarin red S, hASC human adipose-derived stem cell, OC osteocalcin, OM osteogenic media, OSX osterix, PM proliferation media, RUNX2 runt-related transcription factor 2