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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Protein deubiquitinase USP7 is required for osteogenic differentiation of human adipose-derived stem cells

Fig. 5

Knockdown of USP7 or USP7 inhibitor HBX 41,108 application suppresses osteogenic differentiation of hBMMSCs. a Images of ALP staining on day 7, and AZR staining on day 14 of hBMMSCs expressing shNC, shUSP7-1, or shUSP7-2 after osteoinduction. b ALP activity on day 7 and quantification of AZR staining on day 14 of hBMMSCs expressing shNC, shUSP7-1, or shUSP7-2 after osteoinduction. c Images of ALP staining on day 7, and AZR staining on day 14 of hBMMSCs cultured in the absence or presence of HBX 41,108 after osteoinduction. d ALP activity on day 7 and quantification of AZR staining on day 14 of hBMMSCs cultured in the absence or presence of HBX 41,108 after osteoinduction. Results are presented as the mean ± SD, n = 3. *P < 0.05, **P < 0.01. ALP alkaline phosphatase, AZR alizarin red S, hBMMSC human bone marrow-derived mesenchymal stem cell, NC negative control, OM osteogenic media, PM proliferation media, USP7 ubiquitin specific protease 7

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Stem Cell Research & Therapy

ISSN: 1757-6512