Skip to main content
Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Epigenetic reprogramming converts human Wharton’s jelly mesenchymal stem cells into functional cardiomyocytes by differential regulation of Wnt mediators

Fig. 5

In vivo transplantation and engraftment of WJMSCs and CPs, and restoration of cardiac damage. Schematic 2: graphical representation of the induction of cardiotoxicity by doxorubicin in mouse and the engraftment of MSC-derived cardiomyocytes to restore the damaged cardiac tissue. a–d Induction of cardiac fibrosis in mice by treatment with doxorubicin. H&E images of (a) untreated mouse heart without fibrosis and (b) doxorubicin-treated (2.5 mg/kg body weight in six equal injections on alternative days) mouse heart indicating fibrotic regions as shown in the red circle (scale bars = 100 μm, n = 4). Masson’s trichrome staining of (c) control mouse heart (scale bar, 200 μm) and (d) doxorubicin-treated mouse heart showing the heart cross-section (scale bar = 200 μm (left) and scale bar = 50 μm (right)) indicating clearly increased fibrosis (blue square) in the cardiac muscle compared to the control group (n = 4). eg Transplantation of WJMSCs and cardiac progenitors (CP) into mice induced with cardiac fibrosis. (e) Percentage change in body weight, showing a decrease in body weight after the doxorubicin (dox) treatment period. (f) Percentage change in body weight, indicating an increase in body weight in the mice transplanted with CP cells. (g) Representative image of a heart, indicating necrosis in plain medium-treated control, reduced necrotic area in mice transplanted with undifferentiated MSC, and negligible necrosis in CP-transplanted mice; necrotic regions indicated in dashed circles. WJMSC Wharton’s jelly mesenchymal stem cell, H&E hematoxylin and eosin (Color figure online)

Back to article page