Fig. 3

Expression of pluripotency-associated genes in synovium, marrow, and adipose cMSCs. Passage 2 cMSCs were qualitatively evaluated for the pluripotency-associated genes NANOG, OCT4, and SOX2 using RT-PCR. All 15 cMSC cell preparations were positive when assessed using RT-PCR. Representative images of NANOG (274 bp), OCT4 (141 bp), and SOX2 (142 bp) gene expression from synovium, marrow, and adipose-derived cMSCs of a single donor are shown. Canine GAPDH was used as a housekeeping gene. bp base pairs