Fig. 5

Adipogenesis of synovium, marrow, and adipose of cMSCs. a Passage 2 cMSCs were cultured in quadruplicate wells in CCM or modified adipogenic media with media exchange twice weekly. At 21 days, cells were formalin fixed and evaluated for lipid accumulation with Oil Red O (bar = 25 μm). b Oil Red O quantification (mean ± SD) for a representative donor. **Significantly different Oil Red O quantification between treatment conditions (p < 0.01). c Mean ± SD Oil Red O quantification for all 15 cMSC isolates. CCM values have been subtracted from adipogenic values to facilitate data presentation. Data are reported in descending order for each tissue. For b and c, a, b denote significant differences between tissue sources of cMSCs (p < 0.001). CCM complete culture medium