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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Multipotent stromal cells/mesenchymal stem cells and fibroblasts combine to minimize skin hypertrophic scarring

Fig. 2

MSCs plated on a matrix provide a survival advantage for fibroblasts. a MSCs ± fibroblasts were plated with or without tenascin-C matrix on indicated surfaces and then subjected to a 12-hour treatment with FasL. Fluorochrome inhibitor of caspase activity (FLICA red)-stained MSCs and fibroblasts (tracked green) were visualized to monitor apoptosis. Counterstaining is shown in blue with DAPI. b Quantitative analysis using MetaMorph analysis of positive staining. Apoptosis was assessed in comprehensive cocultures in normal media or in no serum with 0.5 or 0.1 g/L glucose for MSC and fibroblast growth alone and with or without tenascin-C, and HA gel. Apoptosis was measured by TUNEL staining (c), FLICA positivity (d), or Annexin V positivity (e), respectively. *p < 0.05, **p < 0.01, compared to control. Data and images from a representative experiment of three trials, with at least n = 3 per experiment per condition, each with a different cell isolate. Original magnifications, ×100. FB fibroblasts, FLICA fluorescent inhibitor of caspase-3 activity, HA hyaluronic acid, MSC mesenchymal stem cells, TNC tenascin-C (Color figure online)

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