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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Multipotent stromal cells/mesenchymal stem cells and fibroblasts combine to minimize skin hypertrophic scarring

Fig. 3

MSCs–fibroblasts aid in reducing immune response and rejection. a Immunohistochemistry micrographs of matrix ± MSCs, TNC, fibroblasts (FB), and HA were probed for CD3 to assess inflammatory infiltration in the transplant environment after 72 hours. Shown is mean ± s.e.m. with MSCs + TNC + FB + HA being statistically different from MSCs alone. b Quantification of CD3+ cells via counting of sections from a, with examination of at least four fields per mouse. c–e FACS analysis for wound CD3 (T cells), CD11b (pan-leukocytes), and F4/80 (macrophages) in an assessment of wound bed inflammatory infiltrate 72 hours post transplantation, quantified by FlowJo software. *p < 0.05, **p < 0.01, compared to control. Data and images from a representative experiment of three trials, with at least n = 3 per experiment per condition, each with a different cell isolate. HA hyaluronic acid, MSC mesenchymal stem cells, NT no treatment, TNC tenascin-C

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