Skip to main content
Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Multipotent mesenchymal stromal cells enhance insulin secretion from human islets via N-cadherin interaction and prolong function of transplanted encapsulated islets in mice

Fig. 6

Islet survival and function in diabetic mice. Islets (4.5 × 103–5 × 103 IEQ) were transplanted into streptozotocin-induced diabetic mice. Mice were transplanted with free islets (n = 6), with microencapsulated islets (n = 12), or with islets coencapsulated with MSC (n = 10) at IEQ:MSC ratio 1:80. a Glycemia measurements 48 h after transplantation and thereafter twice weekly. Islet graft failure concluded when glucose level was > 20 mmol/L for three consecutive measurements. Dotted line, glucose measurements in 12 mice transplanted with encapsulated islets; dashed line, glucose measurements in 10 mice transplanted with encapsulated islets and MSC. b Kaplan-meier curve representing the % of graft survival. Continuous line, mice transplanted with free islets; dotted line, mice transplanted with microencapsulated islets; dashed line, mice trasplanted with islets coencapsulated with MSC. Mantel–Cox test performed. c, d Beads were retrieved from the peritoneum when mice became diabetic. Sections were immunostained against insulin (red) and vimentin (green) for islets and MSC, respectively. Cell nuclei were stained with Hoechst (blue). c Beads recovered from mice transplanted with islets alone. Only empty beads were retrieved. d Beads recovered from mice transplanted with islets and MSC together still contained some islets. e Intraperitoneal glucose tolerance test performed at day 15 in nontransplanted healthy mice (n = 3, continuous line), in diabetic mice transplanted with alginate-PEG islets (n = 3, dotted line), and in mice transplanted with alginate-PEG islets + MSC (n = 10, dashed line). A single intraperitoneal injection of glucose (2 g/kg) performed at time 0 and glucose monitored at 0, 5, 10, 15, 30, 45, 60, and 120 min thereafter. Values expressed as the mean ± SEM of measurement from at least three mice. Area under the curve is calculated to perform t-test statistical analysis

Back to article page