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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Exosomes secreted by stem cells from human exfoliated deciduous teeth contribute to functional recovery after traumatic brain injury by shifting microglia M1/M2 polarization in rats

Fig. 3

Characterization of SHED-Ex and internalization assay. a Morphologic observation of SHED-Ex by transmission electron microscopy (scale bar = 50 nm). b Surface exosomal markers (CD9, CD63) were analyzed using Western blotting in SHED, exosomes from GW4869-treated SHED, and SHED-Exo. c Flow cytometric analysis showing the presence of CD9, CD63, and CD81 in exosomes derived from SHED. d Immunofluorescence image showing the internalization of CM-Dil-labeled exosomes (red) by microglia (scale bar = 150 μm). e DiI uptake was analyzed by flow cytometry with different incubation times

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