Fig. 2

Proliferation potential of older donor MSCs as a function of CD264⁺ cell content. MSCs from male (circle) and female (triangle) donors ages 45–60 years old are described in the legend to Fig. 1. a Growth curve of representative low (circle) and high (triangle) CD264 cultures. b Doubling time of MSC cultures calculated from the growth curve. c,d Phase-contrast micrographs of stained clonogenic colonies from representative low (c) and high (d) CD264 cultures in tissue culture Petri dishes (10-cm diameter). e Colony-forming efficiency of MSCs. Data are reported as mean ± SEM for n = 3 biological replicates. Nonparametric linear regression lines shown in panels b and e for n = 6 donor cultures