Fig. 8

Co-expression of CD264 with p53 and p21 in MSCs during serial passage. MSCs corresponding to Fig. 6 were surface labeled with anti-CD264-phycoerythrin (PE), fixed, permeabilized, and co-labeled with either anti-p53-Alexa Fluor (AF)488 (a,b) or anti-p21-AF488 (d,e) monoclonal antibodies. a,d Representative bivariate histograms of co-labeled MSCs at select passages (n = 10,000 cells/group). b,e Corresponding percentages of MSCs from each quadrant of the bivariate histograms (n = 3 biological replicates). c,f Schematic of the pattern of expression of CD264 with p53 (c) and p21 (f) during serial passage. Donor age 36 years old. Data are reported as mean ± SEM. *p < 0.05, versus passage (P)3 MSCs