Fig. 7

Laryngeal regeneration after transplantation of laryngeal clonal cells following radiation damage. Immunofluorescent staining for collagen I, E-cadherin, AQP4, and AQP5 performed on irradiated rat VFs. At least three random tissue sections per VF from five rats were chosen for evaluation. Scale bars, 20 μm. Fluorescence intensity and epithelial thickness measured using ImageJ. Error bars, SD (n = 5 animals). One-way ANOVA, Tukey’s post-hoc test. *P < 0.05, **P < 0.01, ***P < 0.001 vs Normal; ^## P < 0.01, ^### P < 0.001 vs Vehicle; ^$$$ P < 0.001 vs EM; ^† P < 0.05, ^†† P < 0.01 vs LP. Refer to Additional file 1 for complete statistical values. RT radiation, EM epiglottic mucosa, LP lamina propria, MF macula flava, COL1 collagen I, E-cad Ecadherin, AQP aquaporin