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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Engineering human ventricular heart muscles based on a highly efficient system for purification of human pluripotent stem cell-derived ventricular cardiomyocytes

Fig. 7

Generation and histological analysis of the human ventricular heart muscles. a Process of decellularization of rat hearts and preparation of natural heart ECM. b Major ECM compositions (laminin, fibronectin, and collagen III) of decellularized hearts and native rat hearts detected by immunofluorescence staining. c Schematic diagram of the preparation of engineered human heart muscles using MLC-2v-positive ventricular cardiomyocytes. d Representative image of the engineered human ventricular heart muscles in a light microscope. Scale bar, 200 μm. H&E staining (e) and coimmunostaining by anti-MLC-2v and anti-collagen III antibodies (f) of sections of constructed ventricular heart muscles. hPSC-derived ventricular cardiomyocytes are well distributed and attached on the native ECM. Scale bar, 100 μm. g Double immunofluorescence staining for cTnT/α-smooth muscle actin (α-SMA), cTnT/von Willebrand factor (vWF), and cTnT/CX43 in the engineered human ventricular heart muscles. Scale bars, 100 μm. ECM extracellular matrix, MLC-2v cardiac ventricular isoform of myosin light chain-2, hMSCs human mesenchymal stem cells

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