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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Upregulation of sodium taurocholate cotransporter polypeptide during hepatogenic differentiation of umbilical cord matrix mesenchymal stem cells facilitates hepatitis B entry

Fig. 1

Hepatogenic differentiation of UCMSCs. a CYP3A4 mRNA expression is significantly induced in differentiated umbilical cord mesenchymal stem cells (D-UCMSCs). Values are expressed as fold increase (669 ± 71 SD; n = 3 vs. control UCMSCs (n = 3). Gene expression was normalized by POP4 and TBP housekeeping genes (p = 0.005; Mann-Whitney U test). b Upregulation of CYP3A4 protein expression in D-UCMSCs is demonstrated by Western blot; 90 μg of total protein extracts were loaded per well. Actin was used as the loading control. The blot is representative of three different donors tested. c CYP3A4 activity was also enhanced as demonstrated by using Luciferase assay in D-UCMSCs (1069 ± 157 SD, n = 16) when compared to UCMSCs (CTRL; 153 ± 32 SD, n = 8; p < 0.0001 by Mann-Whitney U test). Values are expressed as the relative light unit (RLU) net signal

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Stem Cell Research & Therapy

ISSN: 1757-6512