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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Upregulation of sodium taurocholate cotransporter polypeptide during hepatogenic differentiation of umbilical cord matrix mesenchymal stem cells facilitates hepatitis B entry

Fig. 2

HBV infection kinetics in D-UCMSCs. a Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) kinetic: infected D-UCMSC samples were collected during 4 days post-infection and were analyzed by ddPCR multiplex assay. HBV cccDNA biogenesis was detectable in D-UCMSCs starting at day 1 up to day 4 post-infection (n = 3). The cell copy number was calculated by RNase P genome detection. Absolute values are expressed as HBV cccDNA copies per cell (donors 112, 114, and 116). b HBV pgRNA detection: viral transcripts (pregenomic (pg) and preC RNAs) were detected by qPCR during HBV infection kinetic in D-UCMSCs. Viral RNAs were detected starting at day 1 and expressed as fold-increase in time as compared to cells infected after 24 h after primary infection (donors 112 and 116). Relative quantification analysis of HBV pg/pc RNAs was normalized by using TBP and POP4 reference genes. c HBV relaxed circular (RC) DNA virion secretion: D-UCMSC infection kinetic studies show an increased production of HBV infectious particles over 4 days (p < 0.05). Absolute quantification of HBV RC DNA positive virions is expressed as fold change versus cells 24 hours after primary infection (donors 112 and 116)

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