Fig. 5

Inhibition of HBV entry in D-UCMSCs. a Inhibition of viral entry by taurocholate (TC) treatment: infected D-UCMSCs were treated 2 h prior to and during infection with different concentrations of TC. Supernatant was collected at day 4. Secreted HBV relaxed circular (RC) DNA virions were quantified by absolute qPCR assay. Values are expressed as absolute copys numbers of HBV RC DNA infectious virions at different TC concentrations and produced by three different donors. b Infected D-UCMSC TC treatment relative to non-treated: treated cells as compared to non-treated cells present a substantial decrease in HBV production when increasing TC concentrations are used (n = 3). Absolute quantification of HBV RC DNA positive virions is expressed as fold increase versus cells 24 h after primary infection