Fig. 4

Human cortical pyramidal-shaped neurons are induced by BMF combination. a Pyramidal morphology index (PMI) and soma size of induced cortical (iCtx) cells compared to human fetal primary cortical (hCtx) cells. Data are shown as mean ± SEM (n = 3). b Tracing showing the pyramidal shape and complex morphology of the iCtx cells which are different from the human striatal (hStr) cells but resembling those of the hCtx cells. c–k Orthogonal projections of confocal images showing expression of the deep-layer cortical neuronal markers SATB2 (c–e), CTIP2 (f-h). and TBR1 (i–k) by iCtx cells, labeled with green fluorescent protein (GFP) under human Synapsin-I promoter. Scale bar = 20 μm