Fig. 6

Human BMF-derived iCtx cells form synaptic contacts with human fetal cortical neurons. a iCtx cells (GFP⁺ and MAP2⁺), co-cultured with hCtx cells (MAP2⁺), have spines in their neurites. Enlarged images of the spines (red arrowheads). Scale bars = 20 μm and 1 μm for the iCtx cell and the enlargements, respectively. b SynI-RFP⁺ iCtx cells express PSD95 in their neurites (arrows). Arrowheads indicate spines. White boxes indicate enlarged neurites. Scale bars = 20 μm and 1 μm for enlargements. c Whole-cell patch clamp recordings from iCtx cells co-cultured with hCtx cells. Current traces show generation of spontaneous PSCs (sPSCs) (i). Isolated glutamatergic sPSCs were recorded in the presence of picrotoxin (Ptx) (ii), and are blocked by D-APV and NBQX (iii). * Expanded fast decaying sPSC shown on the right. d Averaged current trace of fast decaying sPSCs observed under control conditions (Ctr) (i). Time constant (t _decay ) was determined by fitting an exponential (red) curve to the decay. Table summarizes data across experiments (n = 5 cells). e Bar chart summarizing data across experiments (n = 3, one sample t test, p = 0.0017). D-APV and NBQX blocked glutamatergic sPSCs in all cells tested. Data are shown as mean ± SEM