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Table 1 Electrophysiological properties and AP characteristics of induced neuronal cells

From: Direct conversion of human fibroblasts to functional excitatory cortical neurons integrating into human neural networks

 

BMNd

BMF

BMC

BMNgn2

BMNgn2F

BMNgn2C

MNgn2

MNgn2F

MNgn2C

hCtx

Days in vitroa

25–31

26–37

26–38

25–32

27–33

28–34

27–32

27–33

29–34

20–23

Number of coverslips

4

4

5

10

3

3

3

4

4

4

Number of cells

10

11

11

26

9

13

7

9

5

8

Vrest (mV)

–45.3 ± 2.2

–44.1 ± 3.74

–48.3 ± 5.6

–42.6 ± 1.9

–38.6 ± 3.9

–47.4 ± 3.3

–36.9 ± 3.0

–33.3 ± 2.7

–35.3 ± 5.0

–43.9 ± 2.6

Rinput (MΩ)

798 ± 156*

229 ± 29

507 ± 107

686 ± 66*

441 ± 96

623 ± 127

908 ± 120*

800 ± 139*

781 ± 224

644 ± 70

Capacitance (pF)

9.5 ± 1.7

42.0 ± 15.8

25.0 ± 10.7

6.8 ± 1.4*$

35.1 ± 10.2

26.7 ± 10.7

3.0 ± 0.6*$

5.3 ± 2.4*$

3.7 ± 0.8*

6.8 ± 2.1

Cells with more than 1 AP, % (n/N)

80 (8/10)

82 (9/11)

73 (8/11)

65 (17/26)

89 (8/9)

62 (8/13)

43 (3/7)

44 (4/9)

40 (2/5)

75 (6/8)

Number of APs

5.8 ± 1.0*

16.0 ± 3.2

10.6 ± 3.2

6.0 ± 1.2*

5.7 ± 1.5*

7.4 ± 2.7

4.0 ± 1.8*

1.8 ± 0.4*

2.6 ± 1.4*

6.1 ± 1.4

Threshold

–21.4 ± 1.8

–20.5 ± 1.7

–22.0 ± 1.6

–21.2 ± 1.1

–21.6 ± 1.8

–20.6 ± 1.3

–22.6 ± 2.7

–20.7 ± 1.7

–18.8 ± 1.3

–24.5 ± 1.6

Amplitude

39.9 ± 3.4*£

58.5 ± 4.7

60.4 ± 5.4

48.8 ± 2.7£

50.8 ± 5.6

48.6 ± 5.4

44.7 ± 4.1£

39.9 ± 4.4*£

37.3 ± 5.2*£

26.9 ± 7.1

Rise time

1.7 ± 0.3

1.4 ± 0.3

1.6 ± 0.4

1.4 ± 0.1

1.4 ± 0.1

1.4 ± 0.1

1.7 ± 0.2

2.1 ± 0.3

1.6 ± 0.2

1.6 ± 0.2

Half amplitude width

2.3 ± 0.3

2.1 ± 0.4

3.1 ± 0.7

1.7 ± 0.1

1.9 ± 0.3

1.6 ± 0.1

2.0 ± 0.4

2.1 ± 0.2

2.0 ± 0.3

2.2 ± 0.3

After hyperpolarization peak

21.3 ± 3.1

23.5 ± 1.9

18.2 ± 2.8

17.9 ± 1.1

18.2 ± 2.0

19.0 ± 2.5

16.2 ± 3.9

15.7 ± 2.4

18.4 ± 3.8

9.0 ± 2.7

  1. a Day 0 corresponds to the first day after induction
  2. Resting membrane potential (Vrest), one-way ANOVA p = 0.0554
  3. Input resistance (Rinput), Kruskal-Wallis test p = 0.0016, Dunn’s multiple comparison test p < 0.05
  4. Capacitance, Kruskal-Wallis test p < 0.0001, Dunn’s multiple comparison test p < 0.05.
  5. Number of action potentials (APs), one-way ANOVA p = 0.0011, Turkey’s multiple comparisons test p < 0.05. F
  6. AP threshold, Kruskal-Wallis test. p = 0.8026
  7. AP amplitude, one-way ANOVA p = 0.0154, uncorrected Fisher’s LSD p < 0.05.
  8. AP rise time (0–100%), Kruskal-Wallis test p = 0.1343
  9. Half AP amplitude width, Kruskal-Wallis test p = 0.3701
  10. After hyperpolarization peak, Kruskal-Wallis test p = 0.5411
  11. * Statistical difference from B; $ statistical difference from BMNgn2F; £ statistical significance from BMC