| BMNd | BMF | BMC | BMNgn2 | BMNgn2F | BMNgn2C | MNgn2 | MNgn2F | MNgn2C | hCtx |
---|---|---|---|---|---|---|---|---|---|---|
Days in vitroa | 25–31 | 26–37 | 26–38 | 25–32 | 27–33 | 28–34 | 27–32 | 27–33 | 29–34 | 20–23 |
Number of coverslips | 4 | 4 | 5 | 10 | 3 | 3 | 3 | 4 | 4 | 4 |
Number of cells | 10 | 11 | 11 | 26 | 9 | 13 | 7 | 9 | 5 | 8 |
Vrest (mV) | –45.3 ± 2.2 | –44.1 ± 3.74 | –48.3 ± 5.6 | –42.6 ± 1.9 | –38.6 ± 3.9 | –47.4 ± 3.3 | –36.9 ± 3.0 | –33.3 ± 2.7 | –35.3 ± 5.0 | –43.9 ± 2.6 |
Rinput (MΩ) | 798 ± 156* | 229 ± 29 | 507 ± 107 | 686 ± 66* | 441 ± 96 | 623 ± 127 | 908 ± 120* | 800 ± 139* | 781 ± 224 | 644 ± 70 |
Capacitance (pF) | 9.5 ± 1.7 | 42.0 ± 15.8 | 25.0 ± 10.7 | 6.8 ± 1.4*$ | 35.1 ± 10.2 | 26.7 ± 10.7 | 3.0 ± 0.6*$ | 5.3 ± 2.4*$ | 3.7 ± 0.8* | 6.8 ± 2.1 |
Cells with more than 1 AP, % (n/N) | 80 (8/10) | 82 (9/11) | 73 (8/11) | 65 (17/26) | 89 (8/9) | 62 (8/13) | 43 (3/7) | 44 (4/9) | 40 (2/5) | 75 (6/8) |
Number of APs | 5.8 ± 1.0* | 16.0 ± 3.2 | 10.6 ± 3.2 | 6.0 ± 1.2* | 5.7 ± 1.5* | 7.4 ± 2.7 | 4.0 ± 1.8* | 1.8 ± 0.4* | 2.6 ± 1.4* | 6.1 ± 1.4 |
Threshold | –21.4 ± 1.8 | –20.5 ± 1.7 | –22.0 ± 1.6 | –21.2 ± 1.1 | –21.6 ± 1.8 | –20.6 ± 1.3 | –22.6 ± 2.7 | –20.7 ± 1.7 | –18.8 ± 1.3 | –24.5 ± 1.6 |
Amplitude | 39.9 ± 3.4*£ | 58.5 ± 4.7 | 60.4 ± 5.4 | 48.8 ± 2.7£ | 50.8 ± 5.6 | 48.6 ± 5.4 | 44.7 ± 4.1£ | 39.9 ± 4.4*£ | 37.3 ± 5.2*£ | 26.9 ± 7.1 |
Rise time | 1.7 ± 0.3 | 1.4 ± 0.3 | 1.6 ± 0.4 | 1.4 ± 0.1 | 1.4 ± 0.1 | 1.4 ± 0.1 | 1.7 ± 0.2 | 2.1 ± 0.3 | 1.6 ± 0.2 | 1.6 ± 0.2 |
Half amplitude width | 2.3 ± 0.3 | 2.1 ± 0.4 | 3.1 ± 0.7 | 1.7 ± 0.1 | 1.9 ± 0.3 | 1.6 ± 0.1 | 2.0 ± 0.4 | 2.1 ± 0.2 | 2.0 ± 0.3 | 2.2 ± 0.3 |
After hyperpolarization peak | 21.3 ± 3.1 | 23.5 ± 1.9 | 18.2 ± 2.8 | 17.9 ± 1.1 | 18.2 ± 2.0 | 19.0 ± 2.5 | 16.2 ± 3.9 | 15.7 ± 2.4 | 18.4 ± 3.8 | 9.0 ± 2.7 |