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Table 1 Electrophysiological properties and AP characteristics of induced neuronal cells

From: Direct conversion of human fibroblasts to functional excitatory cortical neurons integrating into human neural networks

  BMNd BMF BMC BMNgn2 BMNgn2F BMNgn2C MNgn2 MNgn2F MNgn2C hCtx
Days in vitroa 25–31 26–37 26–38 25–32 27–33 28–34 27–32 27–33 29–34 20–23
Number of coverslips 4 4 5 10 3 3 3 4 4 4
Number of cells 10 11 11 26 9 13 7 9 5 8
Vrest (mV) –45.3 ± 2.2 –44.1 ± 3.74 –48.3 ± 5.6 –42.6 ± 1.9 –38.6 ± 3.9 –47.4 ± 3.3 –36.9 ± 3.0 –33.3 ± 2.7 –35.3 ± 5.0 –43.9 ± 2.6
Rinput (MΩ) 798 ± 156* 229 ± 29 507 ± 107 686 ± 66* 441 ± 96 623 ± 127 908 ± 120* 800 ± 139* 781 ± 224 644 ± 70
Capacitance (pF) 9.5 ± 1.7 42.0 ± 15.8 25.0 ± 10.7 6.8 ± 1.4*$ 35.1 ± 10.2 26.7 ± 10.7 3.0 ± 0.6*$ 5.3 ± 2.4*$ 3.7 ± 0.8* 6.8 ± 2.1
Cells with more than 1 AP, % (n/N) 80 (8/10) 82 (9/11) 73 (8/11) 65 (17/26) 89 (8/9) 62 (8/13) 43 (3/7) 44 (4/9) 40 (2/5) 75 (6/8)
Number of APs 5.8 ± 1.0* 16.0 ± 3.2 10.6 ± 3.2 6.0 ± 1.2* 5.7 ± 1.5* 7.4 ± 2.7 4.0 ± 1.8* 1.8 ± 0.4* 2.6 ± 1.4* 6.1 ± 1.4
Threshold –21.4 ± 1.8 –20.5 ± 1.7 –22.0 ± 1.6 –21.2 ± 1.1 –21.6 ± 1.8 –20.6 ± 1.3 –22.6 ± 2.7 –20.7 ± 1.7 –18.8 ± 1.3 –24.5 ± 1.6
Amplitude 39.9 ± 3.4*£ 58.5 ± 4.7 60.4 ± 5.4 48.8 ± 2.7£ 50.8 ± 5.6 48.6 ± 5.4 44.7 ± 4.1£ 39.9 ± 4.4*£ 37.3 ± 5.2*£ 26.9 ± 7.1
Rise time 1.7 ± 0.3 1.4 ± 0.3 1.6 ± 0.4 1.4 ± 0.1 1.4 ± 0.1 1.4 ± 0.1 1.7 ± 0.2 2.1 ± 0.3 1.6 ± 0.2 1.6 ± 0.2
Half amplitude width 2.3 ± 0.3 2.1 ± 0.4 3.1 ± 0.7 1.7 ± 0.1 1.9 ± 0.3 1.6 ± 0.1 2.0 ± 0.4 2.1 ± 0.2 2.0 ± 0.3 2.2 ± 0.3
After hyperpolarization peak 21.3 ± 3.1 23.5 ± 1.9 18.2 ± 2.8 17.9 ± 1.1 18.2 ± 2.0 19.0 ± 2.5 16.2 ± 3.9 15.7 ± 2.4 18.4 ± 3.8 9.0 ± 2.7
  1. a Day 0 corresponds to the first day after induction
  2. Resting membrane potential (Vrest), one-way ANOVA p = 0.0554
  3. Input resistance (Rinput), Kruskal-Wallis test p = 0.0016, Dunn’s multiple comparison test p < 0.05
  4. Capacitance, Kruskal-Wallis test p < 0.0001, Dunn’s multiple comparison test p < 0.05.
  5. Number of action potentials (APs), one-way ANOVA p = 0.0011, Turkey’s multiple comparisons test p < 0.05. F
  6. AP threshold, Kruskal-Wallis test. p = 0.8026
  7. AP amplitude, one-way ANOVA p = 0.0154, uncorrected Fisher’s LSD p < 0.05.
  8. AP rise time (0–100%), Kruskal-Wallis test p = 0.1343
  9. Half AP amplitude width, Kruskal-Wallis test p = 0.3701
  10. After hyperpolarization peak, Kruskal-Wallis test p = 0.5411
  11. * Statistical difference from B; $ statistical difference from BMNgn2F; £ statistical significance from BMC