Skip to main content
Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Overexpression of hypoxia-inducible factor 1 alpha improves immunomodulation by dental mesenchymal stem cells

Fig. 1

Overexpression of HIF-1 alpha and mitochondrial stress test in MSCs. a Representative Western blot of hypoxia-inducible factor-1 (HIF-1) alpha in mesenchymal stem cells (MSCs) and HIF-MSCs. Loading control was performed with tubulin and levels of HIF-1 alpha protein expression were quantified by densitometry (right panel). b Expression of the HIF-1 alpha mRNA determined by quantitative PCR. GNB2L1 was used as endogenous control. Date represent expression in HIF-MSCs relative to MSCs control (fold change; FC). Quantification of four independent experiments; data are represented as mean ± SE (Student t test, *p ≤ 0.05). c Oxygen comsumption rate (OCR) response to oligomycin (Oligo.; 1 μM), carbonyl cyanide p-trifluoromethoxy-phenylhydrazone (FCCP; 1 μM), and antimycin A/rotenone (Ant.A/Rot.; both 1 μM) injections. HIF-MSCs (gray line) showed a lower OCR than MSCs (black line). d Mitochondrial substrate utilization in HIF-MSCs (gray) and MSCs (black) measured in terms of basal respiration, ATP production, non-mitochondrial respiration, maximal respiration, proton leak, and spare respiratory capacity. Data were normalized against the total amount of protein. Data are represented as mean ± SE of six independent experiments (Student t test, *p ≤ 0.05, **p ≤ 0.01). GFP green fluorescent protein

Back to article page