Fig. 2

Angiogenic activity and growth factor profile of PlaMSC-CM. a In-vitro angiogenic activity of PlaMSC-CM assessed by an endothelial tube formation assay. Endothelial cell tubes were stained with anti-human CD31 antibody and alkaline phosphatase-conjugated secondary goat anti-mouse IgG antibody after 11 days of culture. Effects of CM on the endothelial tube formation were confirmed in the range between positive (VEGFA) and negative control (suramin). Insets show higher magnification of dotted area to demonstrate architecture of the endothelial tubular network. **P < 0.01 as determined by Dunnett’s test. b Growth factor array for angiogenic and angiostatic factors. Black and white bars show the relative intensity ratios of growth factors found in PlaMSC-CM and BMMSC-CM to that of the positive control, respectively. n = 6. Three independent experiments were performed. D-MEM Dulbecco’s modified Eagle’s medium, BMMSC-CM conditioned medium from MSCs isolated from human bone marrow, PlaMSC-CM conditioned medium from MSCs isolated from human term placental tissue, MSC mesenchymal stem cell, VEGF vascular endothelial growth factor