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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Exosomes of human placenta-derived mesenchymal stem cells stimulate angiogenesis

Fig. 3

PlaMSC-derived exosomes. a The exosome fraction of PlaMSC-CM enriched by filtration and ultracentrifugation, and visualized by TEM with 1.5% uranyl acetate. Scale bar = 500 nm. Inset shows higher-magnification images of the exosomes, and a typical cup-shaped morphology was observed. Scale bar = 100 nm. b Immunoelectron microscopic images showing PlaMSC-exo are positive for CD63 but not for calnexin. CD63 used as positive control and calnexin as negative control. Secondary antibody conjugated with 10-nm gold colloidal particles was used. Scale bar = 100 nm. c Particle size evaluated by DLS. Data show the size of particles in the exosome fraction was approximately 100 nm in diameter. d Western blot analysis of the exosome marker CD9. BMMSC-WCL and HeLa-WCL used as controls. PlaMSC-exo exosomes derived from MSCs isolated from human term placental tissue, MSC mesenchymal stem cell, BMMSC human bone marrow-derived MSC, WCL whole cell lysates

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